AC020-002 Link-A-Light ATTO488 Conjugation Kit (3 labeling reactions, each 100-200 µg)

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  • AC020-002

Product Description for Link-A-Light ATTO488 Conjugation Kit

Product is tested for Labeling.

Properties for Link-A-Light ATTO488 Conjugation Kit

Product Category Labeling Kits & Reagents
Quantity 1 kit
Reaction 3 labeling reactions, each 100-200 µg
Conjugate ATTO488
Applications Labeling
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Link-A-Light Atto488 Conjugation Kit

Datasheet Extract

Add. information Applicable to Western Blotting, ELISA, Immunohistochemistry, Immunofluorescence, and FACS analysis.
Application Conjugation.
Background The Link-A-Light conjugation kit allows Fluorescent conjugations to set up in seconds, simply by adding a solution of the antibody to be labeled to a proprietary lyophilised mixture containing a proprietary activated Fluorescent Ligand (Figure 1).
By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, Link-A-Light technology can be used to label small quantities of protein (see Principle).

Label: Atto488 is one of a new generation of Fluorescent labels, which has been optimised for excitation with an argon laser. It has a strong absorption at 501nm, high fluorescence at 523nm (extinction coefficient 9.0 x10e4 cm-1M-1) and high quantum yield.
Protocols Conjugation Reaction: 
1. Before you add antibody to the Link-A-Light Mix, add 1 µl of Link-A-Light Modifier reagent for each 10 µl of Antibody to be labeled. Mix gently. 
2. Remove the screw cap from the vial of Link-A-Light Mix and pipette the antibody sample (with added Link-A-Light Modifier) directly onto the lyophilised material. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette. 
3. Place the cap back on the vial and leave the vial standing for 3 hours at room temperature (20-25°C). Alternatively, and sometimes more conveniently, conjugations can be set up and left overnight, as the longer incubation time has no negative effect on the conjugate. 
4. After incubating for 3 hours (or more), add 1 µl of Link-A-Light Quencher FD reagent for every 10 µl of antibody used. The conjugate can be used after 30 minutes.
General Readings
  1. The Repetitive Oligopeptide Sequences Modulate Cytopathic Potency but Are Not Crucial for Cellular Uptake of Clostridium difficile Toxin A. Alexandra Olling, Sebastian Goy, Florian Hoffmann, Helma Tatge, Ingo Just, Ralf Gerhard. PloS One (2011)
Storage Storage of Conjugates: For any new conjugate, initial storage at 4°C is recommended. A preservative may be desirable for long-term storage. Other storage conditions (e.g. frozen at -70°C or stored at -20°C with 50% glycerol) may also be satisfactory. The best conditions for any particular conjugate must be determined by experimentation.

Storage of Kit: The kit is shipped at ambient temperature in a tamper-evident polypropylene container.
Store at -20°C upon receipt. Shelf life: One year from despatch.
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