AC046-001 Link-A-Light Avidin Conjugation Kit (3 labeling reactions)

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Product Description for Link-A-Light Avidin Conjugation Kit

Product is tested for Labeling.

Properties for Link-A-Light Avidin Conjugation Kit

Product Category Labeling Kits & Reagents
Quantity 3 x 10 µg
Reaction 3 labeling reactions
Conjugate Avidin
Applications Labeling
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Link-A-Light Avidin Conjugation Kit

Datasheet Extract

Application Conjugation.
Background Link-A-Light is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.
The Link-A-Light conjugation kit allows Avidin conjugations to be set up in seconds, simply by adding a solution of the antibody of the protein to be labeled to a proprietary lyophilised mixture containing a proprietary activated Avidin Ligand (Figure 1).
By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, Link-A-Light technology can be used to label small quantities of protein with 100% recovery (see Principle).
Label: Avidin is a 66kDa tetrameric glycoprotein composed of four identical subunits, which has widespread applications due to its very high affinity to the vitamin Biotin.
Protocols 1. Before you add antibody to the Link-A-Light Mix, add 1 µl of Link-A-Light Modifier reagent for each 10 µl of Antibody to be labeled. Mix gently.
2. Remove the screw cap from the vial of Link-A-Light Mix and pipette the antibody sample (with added Link-A-Light Modifier) directly onto the lyophilised material. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette.
3. Place the cap back on the vial and leave the vial standing for 3 hours at room temperature (20-25°C). Alternatively, and sometimes more conveniently, conjugations can be set up and left overnight, as the longer incubation time has no negative effect on the conjugate.
4. After incubating for 3 hours (or more), add 1 µl of Link-A-Light Quencher FD reagent for every 10 µl of antibody used. The conjugate can be used after 30 minutes.
General Readings 1. Targeting MHC Class I Monomers to Dendritic Cells Inhibits the Indirect Pathway of Allorecognition and the Production of IgG Alloantibodies Leading to Long-Term Allograft Survival. Yakup Tanriver, Kulachelvy Ratnasothy, R. Pat Bucy, Giovanna Lombardi and Robert Lechler.
Journal of Immunology, February 15, 2010 vol. 184 no. 4 1757-1764.
Storage Storage of Conjugates: For any new conjugate, storage at 4°C is recommended. A preservative may be desirable for Long-term storage. Other storage conditions (e.g. Frozen at -70°C or stored at -20°C with 50% Glycerol) may also be satisfactory. The best conditions for any particular conjugate must be determined by experimentation.

Storage of Kit: The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20°C upon receipt.
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