Product Description for Link-A-Light Biotin Conjugation Kit
Product is tested for Labeling.
Properties for Link-A-Light Biotin Conjugation Kit
|Product Category||Labeling Kits & Reagents|
|Reaction||1 labeling reaction, 1-2 mg|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Link-A-Light Biotin Type B Conjugation Kit|
|Add. information||Note: All of the components in the kit are compatible with our range of Link-A-Light one-step conjugation kits. This means that you can do direct from purification to labeling.|
|Background||Link-A-Light is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.
The Link-A-Light conjugation kit allows Biotinylations to be set up in seconds, simply by adding a solution of the antibody to be labeled to a proprietary lyophilised mixture containing a proprietary activated Biotin Ligand (Figure 1).
By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, Link-A-Light technology can be used to label small quantities of protein with 100% recovery (see Principle).
Link-A-Light Biotin has been optimised for two separate applications.
Type A is intended for assays in which a Streptavidin-labeled detection reagent will be used, whilst Type B is optimised for assays in which the biotinylated protein is captured by Streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
Label: The attachment of Biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as Western blotting, Immunohisthochemistry and ELISA. The Biotin in the kit has an extended linker to facilitate molecular interactions.
|Protocols||1. Before you add antibody to the Link-A-Light Mix, add 1 µl of Link-A-Light Modifier reagent for each 10 µl of Antibody to be labeled. Mix gently.
2. Remove the screw cap from the vial of Link-A-Light Mix and pipette the antibody sample (with added Link-A-Light Modifier) directly onto the lyophilised material. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette.
3. Place the cap back on the vial and leave the vial standing for 3 hours at room temperature (20-25°C). Alternatively, and sometimes more conveniently, conjugations can be set up and left overnight, as the longer incubation time has no negative effect on the conjugate.
4. After incubating for 3 hours (or more), add 1 µl of Link-A-Light Quencher FD reagent for every 10 µl of antibody used. The conjugate can be used after 30 minutes.
|Storage||Storage of Conjugates: For any new conjugate, storage at 4°C is recommended. A preservative may be desirable for Long-term storage. Other storage conditions (e.g. Frozen at -70°C or stored at -20°C with 50% Glycerol) may also be satisfactory. The best conditions for any particular conjugate must be determined by experimentation.
Storage of Kit: The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20°C upon receipt.