Product Description for Link-A-Light PE-ATTO594 Conjugation Kit
Properties for Link-A-Light PE-ATTO594 Conjugation Kit
|Product Category||Labeling Kits & Reagents|
|Quantity||1 x 100 µg|
|Reaction||1 labeling reaction|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Link-A-Light PE-Atto594 Conjugation Kit|
|Add. information||Applicable to Western Blotting, ELISA, Immunohistochemistry, Immunofluorescence, and FACS analysis|
|Background||Link-A-Light is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.
The Link-A-Light conjugation kit allows PE-Atto594 Tandem conjugations to set up in seconds, simply by adding a solution of the antibody to be labeled to a proprietary lyophilised mixture containing PE-Atto594 (Figure 1).
By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, Link-A-Light technology can be used to label small quantities of protein for FACS analysis with 100% recovery (see Principle).
Label: PE-Atto594 is a Tandem conjugate. The PE is excited at 488nm and functions as an energy donor for the Atto 594 Fluorescent dye. Energy is transferred from the PE to the Atto594 via Fluorescence Resonance Energy Transfer (FRET). The Atto594 emits the energy received from the PE in the form of long wavelength light at 627nm.
|Protocols||1. Before you add antibody to the Link-A-Light Mix, add 1 µl of Link-A-Light Modifier reagent for each 10 µl of Antibody to be labeled. Mix gently.
2. Remove the screw cap from the vial of Link-A-Light Mix and pipette the antibody sample (with added Link-A-Light Modifier) directly onto the lyophilised material. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette.
3. Place the cap back on the vial and leave the vial standing for 3 hours at room temperature (20-25°C). Alternatively, and sometimes more conveniently, conjugations can be set up and left overnight, as the longer incubation time has no negative effect on the conjugate.
4. After incubating for 3 hours (or more), add 1 µl of Link-A-Light Quencher FD reagent for every 10 µl of antibody used. The conjugate can be used after 30 minutes.
|Storage||Storage of Conjugates: For any new conjugate, storage at 4°C is recommended. A preservative may be desirable for Long-term storage. Other storage conditions may also be satisfactory. The best conditions for any particular conjugate must be determined by experimentation.
Storage of Kit: The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20°C upon receipt.