Product Description for Link-A-Light R-Phycoerythrin (R-PE) Conjugation Kit
Properties for Link-A-Light R-Phycoerythrin (R-PE) Conjugation Kit
|Product Category||Labeling Kits & Reagents|
|Quantity||3 x 10 µg|
|Reaction||3 labeling reactions|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Link-A-Light R-PE Conjugation Kit|
|Background||Link-A-Light is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.
The Link-A-Light conjugation kit allows PE conjugations to set up in seconds, simply by adding a solution of the antibody to be labeled to a proprietary lyophilised mixture containing PE (Figure 1).
By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, Link-A-Light technology can be used to label small quantities of protein for FACS analysis (see Principle).
Label: R-Phycoerythrin (R-PE) is a fluorescent protein from the phycobiliprotein family, and is isolated from red algae. The absorbance spectrum of R-PE covers a broad range of excitation wavelengths, which provides an advantage for multi-color immunofluorescent staining or cell sorting. R-PE is one of the most intensely fluorescent phycobiliprotein having orange fluorescence. It is significantly brighter and more photostable than conventional organic fluorophores and it has a high quantum yield.
|Protocols||1. Before you add antibody to the Link-A-Light Mix, add 1 µl of Link-A-Light Modifier reagent for each 10 µl of Antibody to be labeled. Mix gently.
2. Remove the screw cap from the vial of Link-A-Light Mix and pipette the antibody sample (with added Link-A-Light Modifier) directly onto the lyophilised material. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette.
3. Place the cap back on the vial and leave the vial standing for 3 hours at room temperature (20-25°C). Alternatively, and sometimes more conveniently, conjugations can be set up and left overnight, as the longer incubation time has no negative effect on the conjugate.
4. After incubating for 3 hours (or more), add 1 µl of Link-A-Light Quencher FD reagent for every 10 µl of antibody used. The conjugate can be used after 30 minutes.
|Storage||Storage of Conjugates: For any new conjugate, initial storage at 4°C is recommended. A preservative may be desirable for Long-term storage. Other storage conditions (e.g. Frozen at -70°C or stored at -20°C with 50% glycerol) may also be satisfactory. The best conditions for any particular conjugate must be determined by experimentation.
Storage of Kit: The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20°C upon receipt.