Product Description for Link-A-Light Rhodamine Conjugation Kit
Properties for Link-A-Light Rhodamine Conjugation Kit
|Product Category||Labeling Kits & Reagents|
|Reaction||3 labeling reactions, each up to 200 µg|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Link-A-Light Rhodamine Conjugation Kit|
|Add. information||The Rhodamine used in the Link-A-Light kit has an Absorption peak at 520 and 544 nm. Maximum Emission is obtained when the Rhodamine is excited at 544 nm.|
|Background||Link-A-Light is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.
The Link-A-Light conjugation kit allows Rhodamine conjugations to set up in seconds, simply by adding a solution of the antibody to be labeled to a proprietary lyophilised mixture containing Rhodamine (Figure 1).
By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, Link-A-Light technology can be used to label small quantities of protein (see Principle).
Unlike standard Rhodamine labeling procedures, where a large molar excess of rhodamine is employed, the Link-A-Light Rhodamine kit combines a low molar ratio of Rhodamine:Antibody and high labeling efficiency to eliminate the need for purification steps.
Label: Rhodamine dyes are used extensively in biotechnology applications such as Fluorescence Microscopy, Flow Cytometry, Fluorescence correlation spectroscopy and ELISA. Rhodamine has an Emmission wavelength of 576nm and an Excitation wavelength of 544nm.
|Protocols||1. Before you add antibody to the Link-A-Light Mix, add 1 µl of Link-A-Light Modifier reagent for each 10 µl of Antibody to be labeled. Mix gently.
2. Remove the screw cap from the vial of Link-A-Light Mix and pipette the antibody sample (with added Link-A-Light Modifier) directly onto the lyophilised material. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette.
3. Place the cap back on the vial and leave the vial standing for 3 hours at room temperature (20-25°C). Alternatively, and sometimes more conveniently, conjugations can be set up and left overnight, as the longer incubation time has no negative effect on the conjugate.
4. After incubating for 3 hours (or more), add 1 µl of Link-A-Light Quencher FD reagent for every 10 µl of antibody used. The conjugate can be used after 30 minutes.
|Storage||Storage of Conjugates: For any new conjugate, initial storage at 4°C is recommended. A preservative may be desirable for Long-term storage. Other storage conditions (e.g. Frozen at -70°C or stored at -20°C with 50% glycerol) may also be satisfactory. The best conditions for any particular conjugate must be determined by experimentation.
Storage of Kit: The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20°C upon receipt.