Properties for hsa-let-7f RT-PCR Primer Set
|Product Category||PCR Kits|
|Shipping to||Europe only|
|PDF datasheet||View Datasheet|
|Manufacturer||Cohesion Biosciences Ltd.|
The miRNAs RT-PCR primer set contains a stem-loop RT primer and a pair of specific PCR primer set. Combined with the custom's RT and PCR reagent. It provides a sensitive and specific method of real-time PCR for quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
Description: Stem-loop RT primer and PCR primer set to detect mature miRNA hsa-let-7f expression.
PCR Method: 2-Step qRT-PCR
Detection Range: Seven orders of magnitude
Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA
Sample Types: miRNA, Total RNA
Platform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
|Storage||Shipped at 4°C. Store at -20°C for 2 years.|