hsa-mir-24-3p Real-Time RT-PCR Detection and U6 Calibration Kit - CPK2033-50rxns

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Properties for hsa-mir-24-3p Real-Time RT-PCR Detection and U6 Calibration Kit

Application	Real-time RT-PCR The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. Description: Detect mature miRNA hsa-mir-24-3p expression normalized by U6 snRNA. PCR Method: 2-Step qRT-PCR Detection Range: Seven orders of magnitude Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA Sample Types: miRNA, Total RNA Platform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480 Components: 50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
Storage	Shipped at 4°C. Store at -20°C for 6 months.