CPK3218-1nmol hsa-mir-499 RT-PCR Primer Set

Search for all "hsa-mir-499 RT-PCR Primer Set"


Properties for hsa-mir-499 RT-PCR Primer Set

Product Category PCR Kits
Quantity 1 nmol
Reactivity Hu
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer Cohesion Biosciences Ltd.

Datasheet Extract

Application RT-PCR
Real-time RT-PCR
The miRNAs RT-PCR primer set contains a stem-loop RT primer and a pair of specific PCR primer set. Combined with the custom's RT and PCR reagent. It provides a sensitive and specific method of real-time PCR for quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
Description: Stem-loop RT primer and PCR primer set to detect mature miRNA hsa-mir-499 expression.
PCR Method: 2-Step qRT-PCR
Detection Range: Seven orders of magnitude
Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA
Sample Types: miRNA, Total RNA
Platform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
Storage Shipped at 4°C. Store at -20°C for 2 years.
  • LinkedIn