GTX14635 A431 Lysate EGF treated

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0.1 mg / €220.00


Properties for A431 Lysate EGF treated

Product Category Lysates
Quantity 0.1 mg
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Background Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor. A431 cells were cultured for 20 minutes in the presence of 50ng/ml EGF. Cells were lysed in modified RIPA buffer (50mM Tris-HCl, pH7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstain, 1mM sodium fluoride) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% brophenol blue).
Concentration 1 mg/ml
Storage Keep as concentrated solution. Aliquot and store at -20
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