GTX30595 HeLa whole cell extract

Search for all "HeLa whole cell extract"

0.15 mg / €270.00


Properties for HeLa whole cell extract

Product Category Lysates
Quantity 0.15 mg
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Application WB
Background The cells were grown in DMEM supplemented with 10% FBS. Washed cells are then incubated on ice in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 0.1% SDS, 1.0% NP-40, 0.5% sodium deoxycholic acid. Protein integrity is ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.5 mM AEBSF HCl, 0.4 mM Aprotinin, 25 uM Bestatin, 7.5 uM E-64, 10 uM Leupeptin Hemisulfate and 5 uM Pepstatin A).
Concentration 5 mg/ml
Storage Store at -80
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