Properties for HeLa whole cell lysate (Positive control)
|Shipping to||Not USA/Canada|
|PDF datasheet||View Datasheet|
|Manufacturer||Novus Biologicals Inc.|
|Add. information||Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating.
The recommended loading volume per lane is 0.01-0.03 ml depending on the size format of your gel.
|Background||This whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.|
|Storage||Store at -80C. Avoid freeze-thaw cycles.|