Properties for HL60 Lysate
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Application||Western Blot - 10µg to 20µg per lane is recommended for mini gels.|
|Background||HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies. The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro. Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cells. The HL60 cell genome contains an amplified c-myc proto-oncogene; c-myc mRNA levels are correspondingly high in undifferentiated cells but decline rapidly following induction of differentiation.|
|Storage||Store at 2-8°C for continuous use or (in aliquots) at -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: 6 month from despatch.