AL00009PU-S HL60 Lysate

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Properties for HL60 Lysate

Product Category Lysates
Quantity 0.1 mg
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Application Western Blot - 10µg to 20µg per lane is recommended for mini gels.
Background HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies. The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro. Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cells. The HL60 cell genome contains an amplified c-myc proto-oncogene; c-myc mRNA levels are correspondingly high in undifferentiated cells but decline rapidly following induction of differentiation.
Concentration 2mg/ml
Storage Store at 2-8°C for continuous use or (in aliquots) at -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: 6 month from despatch.
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