Properties for Human NALP3 transfected/Untransfected Cell Lysate
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Application||HEK293 cells were transfected with full-length Human NALP3.
Load 10 μl/lane of a 12 well mini-gel.
This transfected cell lysate can be used as a Positive Western blot Control for NALP3 Antibody Cat.-No AP22017PU-N.
|Background||NACHT, LRR and PYD domains-containing protein 3 belongs to the NLRP family and it contains one DAPIN domain, seven LRR (leucine-rich) repeats and one NATCH domain. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation, the immune response and apoptosis. It is also reported that the NLRP3 inflammasome can be activated by pathogen-associated molecular patterns (PAMPs) or endogenous danger-associated molecular patterns (DAMPs).
The activation of the NLRP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multi-system inflammatory disease (NOMID).
|Storage||They are shipped on blue ice and can remain at –20°C for up to 3 months.
For long term storage, keep lysates in dark at –70°C.
Avoid repetitive freeze-thaw cycles, as this may result in degradation of protein and less
defined bands when separated by SDS-PAGE.