discontinued

GTX23871 MCF-7 whole cell lysate

Search for all "MCF-7 whole cell lysate"

0.1 mg / €200.00

GTX23871

Properties for MCF-7 whole cell lysate

Product Category Lysates
Quantity 0.1 mg
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Application WB
Background MCF7 whole cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF, 5 ug/ml Aprotinin, 1 ug/ml Pepstatin-A, 2 ug/ml Leupeptin, 1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Concentration 2 mg/ml
Storage Keep as concentrated solution. Aliquot and store at -20
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