GTX23876 Mouse intestine tissue lysate

Search for all "Mouse intestine tissue lysate"

0.1 mg / €280.00

Properties for Mouse intestine tissue lysate

Product Category Lysates
Quantity 0.1 mg
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Application WB
Background Mouse intestinal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF, 5 microgram/ml Aprotinin, 1 microgram/ml Pepstatin-A, 2 microgram/ml Leupeptin,1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Concentration 2 mg/ml
Storage Stable for 3 months if stored at -20
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