AL00199SU-N Mouse NALP3 transfected/Untransfected Cell Lysate

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Properties for Mouse NALP3 transfected/Untransfected Cell Lysate

Product Category Lysates
Quantity 50 µl
Synonyms Lysates
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Application HEK293 cells were transfected with full-length Mouse NALP3.
Load 10 μl/lane of a 12 well mini-gel.
This transfected cell lysate can be used as a Positive Western blot Control for NALP3 Antibody Cat.-No AP22017PU-N.
Background NACHT, LRR and PYD domains-containing protein 3 belongs to the NLRP family and it contains one DAPIN domain, seven LRR (leucine-rich) repeats and one NATCH domain. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation, the immune response and apoptosis. It is also reported that the NLRP3 inflammasome can be activated by pathogen-associated molecular patterns (PAMPs) or endogenous danger-associated molecular patterns (DAMPs).
The activation of the NLRP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multi-system inflammatory disease (NOMID).
Storage They are shipped on blue ice and can remain at –20°C for up to 3 months.
For long term storage, keep lysates in dark at –70°C.
Avoid repetitive freeze-thaw cycles, as this may result in degradation of protein and less
defined bands when separated by SDS-PAGE.
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