discontinued

GTX24025 Mouse skin normal tissue lysate

Search for all "Mouse skin normal tissue lysate"

0.1 mg / €290.00

GTX24025

Properties for Mouse skin normal tissue lysate

Product Category Lysates
Quantity 0.1 mg
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Application WB
Background Mouse skin tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylene diamine tetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.
Concentration 2 mg/ml
Storage Upon receipt - Store at -20
  • LinkedIn