L034W1 Mouse spleen tissue lysate (denatured)

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0.2 mg / €200.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Properties for Mouse spleen tissue lysate (denatured)

Product Category Lysates
Quantity 0.2 mg
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer Abnova Taiwan Corp.
Manufacturer certificates ISO 13485:2003

Datasheet Extract

Add. information Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml and mixed with 5x SDS sample buffer to become 1xSDS sample buffer (2% SDS, 300 mM beta-mercaptoethanol, 50 mM Tris-HCl pH 6.8, 10 % Glycerol, & 0.01% Bromophenol blue) at final 3 mg/ml. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Lysate quality was assured with a 12% SDS-PAGE Gel stained with Commassive brilliant blue.
Application WB
Concentration 3
Storage Store vial at -20°C or -80°C
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