Properties for NIH/3T3 (mouse NIH/Swiss embryo) whole cell lysate (denatured)
|Shipping to||Europe, USA/Canada|
|PDF datasheet||View Datasheet|
|Manufacturer||Abnova Taiwan Corp.|
|Manufacturer certificates||ISO 13485:2003|
|Add. information||Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml and mixed with 5x SDS sample buffer to become 1xSDS sample buffer (2% SDS, 300 mM beta-mercaptoethanol, 50 mM Tris-HCl pH 6.8, 10 % Glycerol, & 0.01% Bromophenol blue) at final 3 mg/ml. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Lysate quality was assured with a 12% SDS-PAGE Gel stained with Commassive brilliant blue.
|Storage||Store vial at -20°C or -80°C|