AL00183PU-N Ovary Lysate

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Properties for Ovary Lysate

Product Category Lysates
Quantity 0.1 mg
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Application Western blot: 10 μg to 20 μg per lane is recommended for mini gel.
Tissue Lysate Preparation:
Human Ovary tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration 2.0 mg/ml
Storage Store at -20°C for 3 months or at -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
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