Properties for Ovary Lysate
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Application||Western blot: 10 μg to 20 μg per lane is recommended for mini gel.
Tissue Lysate Preparation:
Human Ovary tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
|Storage||Store at -20°C for 3 months or at -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.