NBA1-07085 OLLAS / DYKDDDDK Vector

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10 µg / €380.00
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NBA1-07085

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  • NBA1-07085

Properties for OLLAS / DYKDDDDK Vector

Product Category Molecular Biology
Quantity 10 µg
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Research Areas: Epitope Tags
Application Western Blot, Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. More detailed information can be downloaded here: pCMV-SD-OLLAS/DYKDDDDK, Western Blot
Background The pCMV-NS-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.
Concentration 0.5 mg/ml
Storage Store at 4C. Do not freeze.

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