discontinued

AM08004 PIN-4 Antibody Coktail

Search for all "PIN-4 Antibody Coktail"

6 ml / €540.00

AM08004

Properties for PIN-4 Antibody Coktail

Product Category Others
Target Category
Quantity 6 ml
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y237
Add. information Description: This reagent is a cocktail containing the three (3) antibodies described below.

Monoclonal mouse antibody to human P63.
Anti-P63 is a monoclonal mouse antibody, clone 4A4, produced by immunization with a recombinant protein derived from amino acid 1-205 of human p63. The isotype is IgG2a, kappa. This antibody recognizes a 63 kD protein, which is highly expressed in the basal or progenitor layers of many epithelial tissues.

Monoclonal mouse antibody to human cytokeratin (HMW).
The anti-cytokeratin (HMW) is a monoclonal mouse antibody, clone 34βE12, produced from immunization with solubilized keratin extract from human stratum corneum. The isotype is IgG1, kappa. This antibody recognizes keratin polypeptides of 68, 58, 56.5 and 50 kD in stratum corneum extracts. The antibody reacts with squamous, ductal and other complex epithelia.

Rabbit anti-p504S (AMACR) antibody. Anti-p504S (AMACR) is a polyclonal rabbit antibody, produced by immunization with synthetic human AMACR peptide. The antibody comes from a purified immunoglobulin fraction of rabbit antiserum against AMACR. P504S (AMACR) encodes a protein involved in the beta-oxidation of branched chain fatty acids.
Application Immunohistochemistry.
Staining Protocol:
We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Positive ControlProstatic intraepithelial neoplasia (PIN).
Cellular Localization: Nuclear for P63 and Cytoplasmic for both Cytokeratin (HMW) and P504S (AMACR).
Caution This cocktail should be detected with sequential application of UnoVueTM 1-Step Polymer Mouse HRP and UnoVueTM 1-Step Rabbit AP Polymer rather than broad spectrum polymer reagents.
General Readings
  1. Beach et al. Am J Surg Pathol 26 (12): 1588, 2002.
  2. Luo et al. Cancer Res 62 (8): 2220, 2002.
  3. Molinie et al. Mod Pathol 17: 1180, 2004.
  4. Tacha and Miller Appl Immunohistochem Mol Morphol 12 (1): 75, 2004.
  5. Signoretti et al. Am J Pathol 157 (6): 1769, 2000.
  6. Wang et al. Differentiation 68 (4-5): 270, 2001.
  7. Yang et al. Mol Cell 2: 305, 1998.
Storage Store the antibody at 2-8°C
Shelf life: one year from despatch.
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