Properties for PIN-4 Antibody Coktail
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Monoclonal Antibodies (de)|
|Add. information||Description: This reagent is a cocktail containing the three (3) antibodies described below.
Monoclonal mouse antibody to human P63.
Anti-P63 is a monoclonal mouse antibody, clone 4A4, produced by immunization with a recombinant protein derived from amino acid 1-205 of human p63. The isotype is IgG2a, kappa. This antibody recognizes a 63 kD protein, which is highly expressed in the basal or progenitor layers of many epithelial tissues.
Monoclonal mouse antibody to human cytokeratin (HMW).
The anti-cytokeratin (HMW) is a monoclonal mouse antibody, clone 34βE12, produced from immunization with solubilized keratin extract from human stratum corneum. The isotype is IgG1, kappa. This antibody recognizes keratin polypeptides of 68, 58, 56.5 and 50 kD in stratum corneum extracts. The antibody reacts with squamous, ductal and other complex epithelia.
Rabbit anti-p504S (AMACR) antibody. Anti-p504S (AMACR) is a polyclonal rabbit antibody, produced by immunization with synthetic human AMACR peptide. The antibody comes from a purified immunoglobulin fraction of rabbit antiserum against AMACR. P504S (AMACR) encodes a protein involved in the beta-oxidation of branched chain fatty acids.
We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Positive ControlProstatic intraepithelial neoplasia (PIN).
Cellular Localization: Nuclear for P63 and Cytoplasmic for both Cytokeratin (HMW) and P504S (AMACR).
|Caution||This cocktail should be detected with sequential application of UnoVueTM 1-Step Polymer Mouse HRP and UnoVueTM 1-Step Rabbit AP Polymer rather than broad spectrum polymer reagents.|
|Storage||Store the antibody at 2-8°C
Shelf life: one year from despatch.