discontinued

GTX76670 LEUCOPERM

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50 Tests / €240.00

GTX76670

Properties for LEUCOPERM

Product Category Others
Quantity 50 Tests
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Application FACS
Background Reagent A - Fixation mediumReagent B - Permeabilization mediumLEUCOPERM reagents are intended for fixing cells in suspension with Reagent A and then permeabilising the cells with Reagent B. This procedure gives antibodies access to intracellular structures and leaves the morphological scatter characteristics of the cells intact. Specific formulations reduce background staining and allow simultaneous addition of permeabilisation medium and fluorochrome labelled antibodies.Flow cytometric analysis with monoclonal antibodies have been restricted primarily to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies.Also excluded from flow cytometric studies were cytoplasmic localisations of well established membrane molecules such as CD3 and CD22.LEUCOPERMTM reagents allow intracellular antigen analysis with the same ease as surface antigens. The only prerequisite is the availability of suitable antibody conjugates. Most commercially available monoclonal antibody conjugates can be used with LEUCOPERM TM reagents. Some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time may have to be determined experimentally for each antibody conjugate.
Storage Cell Permeabilisation reagents should be stored and used at room temperature. DO NOT FREEZE. Do not use reagents if a precipitate forms or discolouration occurs.
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