501-0015 PiBind Resin

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Properties for PiBind Resin

Product Category Others
Quantity 5 g
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

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Inorganic phosphate (Pi) is a product of many enzymatic reactions, for example, those catalysed by phosphatases and ATPases. Measurement of released Pi allows enzyme activity to be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably high assay backgrounds. PiBind(TM) resin provides a quick and easy way to remove contaminating Pi from buffers. The resin works over a broad range of pH values and is unaffected by many commonly used buffer additives.
PiBind(TM) resin can also be used to remove Pi from protein samples (e.g. tissue extracts). This method is quicker than dialysis and unlike desalting does not necessarily lead to a significant dilution of the sample.
In most applications of PiBind(TM) the resin is added to the contaminated sample or buffer solution which is then gently stirred or agitated. After adsorption of Pi, the resin.


2.1. Preparation of PiBind(TM) resin

The resin is supplied as slurry in water. Upon prolonged storage or after rough handling (e.g. magnetic stirring) the solution above the resin may appear cloudy. In this situation, carefully decant the liquid and replace with distilled water.
Since PiBind(TM) is a dense matrix it settles rapidly, thus each wash step only takes a few minutes. This approach may also be used to exchange the resin into a buffer if required.

2.2. Pipetting of PiBind(TM) resin
Many pipette tips have a fine bore at the point and pipetting of the resin may be difficult, particularly in the 50-200 ml range. This problem is easily overcome by using scissors to remove 3-4 mm of plastic from the end of the tip to increase the bore.
PiBind(TM) resin settles quickly and it is necessary to agitate the solution while dispensing multiple aliquots. Magnetic stirring may be used with caution but gentler methods, such as shaking or overhead stirring, are less damaging to the beads. If any


PiBind resin has an extremely high affinity for inorganic phosphate, and is used to eliminate contaminating Pi from buffer solutions to reduce background signals in phosphatase or ATPase assays.

PiBind resin may also be used to remove Pi from cell and tissue extracts and is a rapid alternative to desalting or dialysis.

Storage Store at 4C. Do not freeze.
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