discontinued

PA1143 6CKine (Exodus-2, TCA-4, SLC, CCL21)

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5 µg / €110.00

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6CKine (Exodus-2, TCA-4, SLC, CCL21)

PA1143

Product Description for 6CKine (Exodus-2, TCA-4, SLC, CCL21)

6CKine (Exodus-2, TCA-4, SLC, CCL21).
Presentation: Purified

Properties for 6CKine (Exodus-2, TCA-4, SLC, CCL21)

Product Category Proteins & Growth Factors
Quantity 5 µg
Synonyms CCL21, Exodus2, TCA4
Presentation Purified
Molecular weight 12 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Endotoxin is less than 0.1 ng/µg (IEU/µg) of 6CKine.
Dimers / aggregates: less than 1%
General Readings 1. Analysis of Antitumor Activity Elicited by Vaccination with Combinations of Interleukin-12 DNA with gp100 DNA or the Chemokine CCL21 In Vivo. Hum Gene Ther 2006 Aug;17(8):859-70
2. The N-terminal domain of CCL21 reconstitutes high affinity binding, G protein activation, and chemotactic activity, to the C-terminal domain of CCL19. Biochem Biophys Res Commun 2006 Sep 29;348(3):1089-93
3. Expression of CCR7 and its ligands CCL19/CCL21 in muscles of polymyositis.
J Neurol Sci 2006 Aug 1.
4. Role of CCL21 and CCL19 in allergic inflammation in the ovalbumin-specific murine asthmatic model. J Allergy Clin Immunol 2006 May;117(5):1040-6
5. Loss of dendritic cell migration and impaired resistance to Leishmania donovani infection in mice deficient in CCL19 and CCL21. J Immunol 2006 May 1;176(9):5486-93
6. Intrapulmonary administration of CCL21 gene-modified dendritic cells reduces tumor burden in spontaneous murine bronchoalveolar cell carcinoma. Can
Storage Lyophilized 6CKinealthough stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution 6CKine should be stored at
4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles. Shelf life: one year from despatch.
Source E. coli
Description
Description:
SLC/CCL21, normally expressed in high endothelial venules and in T cell zones of spleen and lymph nodes, strongly attracts T cells and dendritic cells (DC). Secondary lymphoid tissue chemokine (CCL21/SLC) regulates the homing of naive T cells and dendritic cells that express CC-chemokine receptor 7 (CCR7) from distant sites to lymphoid tissue such as lymph nodes. Recombinant Human Exodus-2 is also known as SLC (Secondary Lymphoid-Tissue Chemokine), TCA-4 (T-cell activation-4) , and 6Ckine. Recombinant CCL21 produced in E. coli as a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12219 Dalton. The sequence of the first five N-terminal amino acids was determined and was found to be, Ser-Asp-Gly-Gly-Ala. Precursor- Protein structure and amino acid sequence: Format: This antigen is supplied as sterile filtered, white lyophilized (freeze-dried) powder. 6CKine was lyophilized from a concentrated (1 mg/ml) solution in water containing no additives. It is recommended to reconstitute the lyophilized 6CKine in sterile 18 MΩ H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Format (continued): Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a standard solution of 6CKine as a Reference Standard.
Format
Purity:
>98% is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Endotoxin is less than 0.1 ng/µg (IEU/µg) of 6CKine.
Dimers / aggregates: less than 1%
State:
Purified
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