discontinued

AR20011PU-N Amphiregulin (98aa)

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Amphiregulin

AR20011PU-N

Product Description for Amphiregulin

Amphiregulin.

Properties for Amphiregulin

Product Category Proteins & Growth Factors
Target Category
Quantity 50 µg
Synonyms AREG, CRDGF, Colorectum cell-derived growth factor, SDGF, Schwannoma-Derived Growth Factor
Molecular weight 11.3 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P15514
Purity > 95 % pure by SDS-PAGE gel and HPLC analyses.
Background Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity.
Storage Store the lyophilized protein at -20°C.
Reconstituted Amphiregulin should be stored in working aliquots
with a carrier protein at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues.
AA Sequence:
SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK
Biological activity:
Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.
Molecular weight:
(98 aa)
Format
Endotoxin level:
< 0.1 ng per μg (1EU/μg).
Purity:
>95% pure by SDS-PAGE gel and HPLC analyses.
State:
Lyophilized (sterile filtered) purified protein.
Reconstitution:
Restore in water containing BSA (50 µg of BSA per 1 µg of protein) to a concentration of 0.1-1.0 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use.
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