AR20030PU-S Arginine Deiminase (ADI)

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5 µg / €140.00
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Arginine Deiminase (ADI)

AR20030PU-S

Product Description for Arginine Deiminase (ADI)

Arginine Deiminase (ADI).
Presentation: Purified

Properties for Arginine Deiminase (ADI)

Product Category Proteins & Growth Factors
Quantity 5 µg
Synonyms AD, Arginine Dihydrolase, arcA
Presentation Purified
Molecular weight 46.3 kDa
Source E. coli
Species (Protein) Mycobacteria
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P23793
Purity > 98 % pure by SDS-PAGE gel and HPLC analyses
Background Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis.
Storage Prior to reconstitution store at 2-8°C for 6 months, at RT for one month 
or at -20°C to -80°C for longer.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids.
AA Sequence:
SVFDSKFKGI HVYSEIGELE SVLVHEPGRE IDYITPARLD ELLFSAILES HDARKEHKQF VAELKANDIN VVELIDLVAE TYDLASQEAK DKLIEEFLED SEPVLSEEHK VVVRNFLKAK KTSRELVEIM MAGITKTDLG IEADHELIVD PMPNLYFTRD PFASVGNGVT IHYMRYKVRQ RETLFSRFVF SNHPKLINTP WYYDPSLKLS IEGGDVFIYN NDTLVVGVSE RTDLQTVTLL AKNIVANKEC EFKRIVAINV PKWTNLMHLD TWLTMLDKDK FLYSPIANDV FKFWDYDLVN GGAEPQPVEN GLPLEGLLQS IINKKPVLIP IAGEGASQME IERETHFDGT NYLAIRPGVV IGYSRNEKTN AALEAAGIKV LPFHGNQLSL GMGNARCMSM PLSRKDVKW
Biological activity:
Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.
Molecular weight:
(409 amino acids)
Format
Buffer System:
2mM Sodium Phosphate, pH 7.5 + 0.2mM DTT
Endotoxin level:
< 0.1 ng per μg (1EU/μg).
Purity:
>98% pure by SDS-PAGE gel and HPLC analyses
State:
Lyophilized (0.2µ Sterile filtered) purified protein
Purified
Reconstitution:
Restore in water to a concentration of 0.1-1.0 mg/ml. Centrifuge the vial prior to opening.  
Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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