RA103F Avidin

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2 mg / €210.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview



Product Description for Avidin

Presentation: FITC

Properties for Avidin

Product Category Proteins & Growth Factors
Target Category
Quantity 2 mg
Synonyms AVD
Presentation FITC
Source Egg white
Species (Protein) Chicken
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Purity (Prepared from chromatographical pure Avidin).
Application This product is designed for Immunofluorescence microscopy, fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA. 1/10,000-1/50,000
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Background The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Modified from The and Feltkamp, Immunology 18; 865, 1970 (Conjugation).
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Avidin Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Avidin. No reaction was observed against anti-Streptavidin.
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (Immunoglobulin and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption/Emission: 495 nm / 528 nm
Fluorochrome/Protein Ratio: 2.0 moles FITC per mole of Avidin
(Prepared from chromatographical pure Avidin).
Restore with 1.0 ml of deionized water (or equivalent).
For extended storage mix product with glycerol to 50%.
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