discontinued

AR10478PU-S Beta Lactamase

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1 mg / €300.00

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Beta Lactamase

AR10478PU-S

Product Description for Beta Lactamase

Beta Lactamase.
Presentation: Purified

Properties for Beta Lactamase

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Molecular weight 29 kDa
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P62593
Purity >90.0% as determined by both RP-HPLC and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.
Background

Beta-lactamase is a type of enzyme(EC3.5.2.6) produced by some bacteria that is responsible for their resistanceto beta-lactam antibioticslike penicillins, cephalosporins, cephamycinsand carbapenems. These antibioticshave a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties.

Concentration 1.0 mg/ml (prior to lyophil.)
Storage Prior to reconstitution store at 2-8°C for one month or desiccated below -18°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Beta-Lactamase produced in E.coli is a single, non-glycosylated polypeptide chain containing 263 amino acids.
AA Sequence:
MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVRELCSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Specific activity:
1,500IU/mg. 
Unit Definition: One unit will hydrolyze 1.0 µmole of indicated substrate per min at pH 7.0 at 25°C. The International Unit (using Benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Format
Buffer System:
Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphate buffer pH 7
Purity:
>90.0% as determined by both RP-HPLC and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.
State:
Sterile Filtered White lyophilized (freeze-dried) powder
Purified
Reconstitution:
Restore in sterile 18MΩ-cm-1 H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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