AR10478PU-S Beta Lactamase

Swiss Prot Num:

P62593

Beta-lactamase is a type of enzyme(EC3.5.2.6) produced by some bacteria that is responsible for their resistanceto beta-lactam antibioticslike penicillins, cephalosporins, cephamycinsand carbapenems. These antibioticshave a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties.

Description:

Recombinant Beta-Lactamase produced in E.coli is a single, non-glycosylated polypeptide chain containing 263 amino acids.

AA Sequence:

MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVRELCSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

Specific activity:

1,500IU/mg. 
Unit Definition: One unit will hydrolyze 1.0 µmole of indicated substrate per min at pH 7.0 at 25°C. The International Unit (using Benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Buffer System:

Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphate buffer pH 7

Purity:

>90.0% as determined by both RP-HPLC and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.

State:

Sterile Filtered White lyophilized (freeze-dried) powder
Purified

Reconstitution:

Restore in sterile 18MΩ-cm^-1 H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.