NB810-61090 cAMP-dependent protein kinase; catalytic subunit PKA

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cAMP-dependent protein kinase; catalytic subunit PKA

Product Description for cAMP-dependent protein kinase; catalytic subunit PKA

cAMP-dependent protein kinase; catalytic subunit PKA.

Properties for cAMP-dependent protein kinase; catalytic subunit PKA

Product Category Proteins & Growth Factors
Quantity 1 µg
Synonyms Bovine Heart, Protein Kinase A
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Background Protein Kinase A (PKA) is the kinase that mediates the majority of the actions of the intracellular second messenger cAMP in diverse tissues and phyla. PKA regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis and ion channel function and it may play a key role in memory formation (Micheau and Riedel, 1999). The purified kinase is suitable for studies of PKA phosphorylation in vitro with purified substrates or following introduction into intact cells or cell extracts (Poisbeau et al., 1999; VanRenterghem et al., 1994; Martin et al., 2004). Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
Concentration 0.02 mg/ml
General Readings
  1. Beavo JA, Bechtel PJ, Krebs EG. Preparation of homogeneous cyclic AMP-dependent protein kinase(s) and its subunits from rabbit skeletal muscle. Methods Enzymol. 1974;38:299-308. PubMed PMID: 4375763.
  2. Martin NP, Whalen EJ, Zamah MA, Pierce KL, Lefkowitz RJ (2004) PKA-mediated phosphorylation of the b1-adrenergic receptor promotes Gs/Gi switching. Cellular Signaling 16:1397-1403.
  3. Micheau J, Riedel G. Protein kinases: which one is the memory molecule? Cell Mol Life Sci. 1999 Apr;55(4):534-48. PubMed PMID: 10357224.
  4. Poisbeau P, Cheney MC, Browning MD, Mody I. Modulation of synaptic GABAA receptor function by PKA and PKC in adult hippocampal neurons. J Neurosci. 1999 Jan 15;19(2):674-83. PubMed PMID: 9880588.
  5. VanRenterghem B, Browning MD, Maller JL. Regulation of mitogen-activated protein kinase activation by protein kinases A and C in a cell-free system. J Biol Chem. 1994 Oct 7;269(40):24666-72. PubMed PMID: 7929138.
Storage Store at -80C. Avoid freeze-thaw cycles.
Buffer System:
No Preservative
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