AR10189PU-N Casein Kinase II Holoenzyme (active kinase)

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Casein Kinase II Holoenzyme


Product Description for Casein Kinase II Holoenzyme

Casein Kinase II Holoenzyme.
Presentation: Purified

Properties for Casein Kinase II Holoenzyme

Product Category Proteins & Growth Factors
Quantity 10 µg
Presentation Purified
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >95.0% as determined by SDS-PAGE
Concentration 0.5 mg/ml

Store the antigen undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Human recombinant Casein Kinase 2 holo enzyme containing alpha and beta subunits which were separately expressed in E. coli as non-fusion proteins and purified using several chromatographic steps. The holo enzyme has been reconstituted in the course of the purification and is highly active suitable for labelling CK2 substrates. CK2 holoenzyme is a non-glycosilated polypeptide having a Molecular Mass of 140 kDa. 
Unit Definition: No protease activity detectable.
Specific activity:
> 1.3U/mg. 
1U = 1μmol/min at 37°C using the Synthetic peptide RRRDDDSDDD.
Buffer System:
25mM Tris-HCl, 500mM NaCl, 1mM DTT, 500 μM PMSF, 5% Glycerol, pH 8.5
>95.0% as determined by SDS-PAGE
Liquid sterile filtered clear solution
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