AR50448PU-N Catalase (1-527, His-tag)

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0.5 mg / €730.00
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Catalase

Product Description for Catalase

Catalase.
Presentation: Purified

Properties for Catalase

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms CAT, Peroxisome Marker
Presentation Purified
Molecular weight 61.9 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P04040
Purity > 90 % by SDS - PAGE
Background CAT is a key antioxidant enzyme in the bodies defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide.
Concentration 1 mg/ml (determined by Bradford assay)
Protocols Activity assay:
1. Prepare an assay buffer with the following concentrations: 50 mM Potassium Phosphate (pH 8.0) and 0.15 % H2O2.
2. Load 150ul assay buffer into each well.
3. Add 50ul of recombinant CAT protein with 0.25ug, 0.125ug to each well.
4. Record the decrease in A260nm for 5 minutes at 25°C using UV plate.

General Readings Freitas MO, et al. (2011) J Biol Chem. 2011 Nov 25;286(47):40509-19. Epub 2011 Oct 5.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human CAT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MADSRDPASD QMQHWKEQRA AQKADVLTTG AGNPVGDKLN VITVGPRGPL LVQDVVFTDE MAHFDRERIP ERVVHAKGAG AFGYFEVTHD ITKYSKAKVF EHIGKKTPIA VRFSTVAGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDPIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HMNGYGSHTF KLVNANGEAV YCKFHYKTDQ GIKNLSVEDA ARLSQEDPDY GIRDLFNAIA TGKYPSWTFY IQVMTFNQAE TFPFNPFDLT KVWPHKDYPL IPVGKLVLNR NPVNYFAEVE QIAFDPSNMP PGIEASPDKM LQGRLFAYPD THRHRLGPNY LHIPVNCPYR ARVANYQRDG PMCMQDNQGG APNYYPNSFG APEQQPSALE HSIQYSGEVR RFNTANDDNV TQVRAFYVNV LNEEQRKRLC ENIAGHLKDA QIFIQKKAVK NFTEVHPDYG SHIQALLDKY NAEKPKNAIH TFVQSGSHLA AREKANL
Molecular weight:
(547aa)
Specific activity:
>30,000 units/mg.
One unit will decompose 1.0 umole of H2O2 per minute at pH 8.0 at 25°C.
(Activity assay see "Protocols").
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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