discontinued

AR20009PU-S CD178 / Fas Ligand (N-term, His-tagged)

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CD178 / Fas Ligand

AR20009PU-S

Product Description for CD178 / Fas Ligand

CD178 / Fas Ligand.

Properties for CD178 / Fas Ligand

Product Category Proteins & Growth Factors
Target Category
Quantity 2 µg
Synonyms APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Molecular weight 17.9 kDa
Source CHO cells
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P48023
Purity > 95 % pure by SDS-PAGE gel and HPLC analyses
Add. information Centrifuge the vial prior to opening!
Background Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells.
Storage Prior to reconstitution store at 2-8°C for 6 months, at RT for one month
or at -20°C to -80°C for longer.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.
AA Sequence:
HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL
Biological activity:
Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤ 10.0 ng/ml
Specific activity:
≥ 1 x 105 units/mg.
Format
Endotoxin level:
< 0.1 ng per μg (1EU/μg)
Purity:
>95% pure by SDS-PAGE gel and HPLC analyses
State:
Lyophilized (0.2µ Sterile filtered) purified protein with no Additives
Reconstitution:
Restore in water to a concentration of 0.1-1.0 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use.
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