AR09019PU-N CD26 / DPP4 (39-766, His-tag)

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100 µg / €370.00
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CD26 / DPP4

AR09019PU-N

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Product Description for CD26 / DPP4

CD26 / DPP4.
Presentation: Purified

Properties for CD26 / DPP4

Product Category Proteins & Growth Factors
Target Category
Quantity 100 µg
Synonyms ADABP, ADCP2, Adenosine deaminase complexing protein 2, DPP IV, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103
Presentation Purified
Molecular weight 86.4 kDa
Source High-5 Insect cells
Specactivity > 50 unit/mg
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P27487
Purity > 95 % by SDS PAGE
Background DPP4, dipeptidyl peptidase-4 is a complex enzyme expressed on the surface of most cell types and is a serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. DPP4 protein is associated with intracellular signal transduction, apoptosis and plays an important role in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1).
Concentration 0.5 mg/ml
General Readings Pratley RE and Salsali A. (2007) Curr Med Res Opin. 23(4):919-31.
Rosenstock J. and Zinman B. (2007) Curr Opin Endocrino Diabetes Obes.60(11):1454-70.
Barnett A. (2006). J.Clin. Pract. 60(11):1454-70.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human DPP4 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques.
AA Sequence:
ADP-R KTYTLTDYLK NTYRLKLYSL RWISDHEYLY KQENNILVFN AEYGNSSVFL ENSTFDEFGH SINDYSISPD GQFILLEYNY VKQWRHSYTA SYDIYDLNKR QLITEERIPN NTQWVTWSPV GHKLAYVWNN DIYVKIEPNL PSYRITWTGK EDIIYNGITD WVYEEEVFSA YSALWWSPNG TFLAYAQFND TEVPLIEYSF YSDESLQYPK TVRVPYPKAG AVNPTVKFFV VNTDSLSSVT NATSIQITAP ASMLIGDHYL CDVTWATQER ISLQWLRRIQ NYSVMDICDY DESSGRWNCL VARQHIEMST TGWVGRFRPS EPHFTLDGNS FYKIISNEEG YRHICYFQID KKDCTFITKG TWEVIGIEAL TSDYLYYISN EYKGMPGGRN LYKIQLSDYT KVTCLSCELN PERCQYYSVS FSKEAKYYQL RCSGPGLPLY TLHSSVNDKG LRVLEDNSAL DKMLQNVQMP SKKLDFIILN ETKFWYQMIL PPHFDKSKKY PLLLDVYAGP CSQKADTVFR LNWATYLAST ENIIVASFDG RGSGYQGDKI MHAINRRLGT FEVEDQIEAA RQFSKMGFVD NKRIAIWGWS YGGYVTSMVL GSGSGVFKCG IAVAPVSRWE YYDSVYTERY MGLPTPEDNL DHYRNSTVMS RAENFKQVEY LLIHGTADDN VHFQQSAQIS KALVDVGVDF QAMWYTDEDH GIASSTAHQH IYTHMSHFIK QCFSLP-SGRLVPRGSHHHHHH
Biological activity:
Approximately > 50 Unit/mg.
One unit will hydrolyze 1 micromole of p-nitroaniline per minute at pH 8.0 at 37°C using 1mM of Gly-Pro p-nitroanilde as a substrate.
Assay procedure and results:
- Reaction buffer: 20mM Tris pH 8.0, 0.1M NaCl, 1mM EDTA
- Total reaction volume: 100 ul
- Reaction temperature: 37°C
1. Add the reaction buffer to each well
2. Add the 10 ul of 10 mM substrate (Gly-Pro p-nitroanilide) to each well
3. Add the enzyme (DPP-4) diluent to each well
4. Incubate the 96 well plate at 37°C.
5. Read the optical density at 405 nm.
(see "Pictures" below)
Molecular weight:
(746aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM EDTA, 10% glycerol
Endotoxin level:
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity:
>95% by SDS PAGE
State:
Liquid purified protein
Purified
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