TP301278 CHK2 (transcript variant 1)

Recombinant protein of human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1

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20 µg / €748.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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CHK2

TP301278

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  • TP301278

Product Description for CHK2

CHK2.

Properties for CHK2

Product Category Proteins & Growth Factors
Target Category
Quantity 20 µg
Synonyms CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Molecular weight 60.7 kDa
Source HEK293 cells
Species (Protein) Human
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O96017
Purity > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
Add. information Tag: C-terminal MYC/DDK
Storage Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
Description
Specific activity:
CHEK2 activity verified in a biochemical assay: CHEK2 (CHK2 checkpoint homolog) (TP301278) activity was measured in a homogeneous time-resolved fluorescent (HTRF?) assay. CHEK2 is a serine/threonine kise that is involved in the control of the cell cycle. Varying concentrations of CHEK2 were added to a reaction mix containing ATP and a biotinylated kise substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent sigl was measured on a Flexstation 3 microplate reader. The time resolved fluorescent sigl is expressed as ?delta R? or ?ΔR? and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Format
Buffer System:
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Purity:
>80%
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
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