AR50220PU-N coaA (1-316, His-tag)

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0.5 mg / €730.00
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Quick Overview

coaA

Product Description for coaA

coaA.
Presentation: Purified

Properties for coaA

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms panK, pantothenate kinase, rts, ts-9
Presentation Purified
Molecular weight 38.9 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A6I3
Purity > 95 % by SDS - PAGE
Background coaA (pantothenate kinase) belongs to the prokaryotic pantothenate kinase family. Pantothenate kinase (PanK; CoaA) is the first enzyme in the Coenzyme A biosynthetic pathway. It phophorylates pantothenate (vitamin B5) to form 4'-phosphopantothenate. Regulation of pantothenate kinase activity by feedback inhibition is the critical factor controlling the intracellular CoA concentration.
Concentration 1mg/ml (determined by Bradford assay)
General Readings Song W.-J., et al. (1992) J.Bacteriol. 174:6411-6417Song W.-J., et al. (1992) J.Bacteriol. 174:1705-1706
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli coaA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMSIKEQ TLMTPYLQFD RNQWAALRDS VPMTLSEDEI ARLKGINEDL SLEEVAEIYL PLSRLLNFYI SSNLRRQAVL EQFLGTNGQR IPYIISIAGS VAVGKSTTAR VLQALLSRWP EHRRVELITT DGFLHPNQVL KERGLMKKKG FPESYDMHRL VKFVSDLKSG VPNVTAPVYS HLIYDVIPDG DKTVVQPDIL ILEGLNVLQS GMDYPHDPHH VFVSDFVDFS IYVDAPEDLL QTWYINRFLK FREGAFTDPD SYFHNYAKLT KEEAIKTAMT LWKEINWLNL KQNILPTRER ASLILTKSAN HAVEEVRLRK
Molecular weight:
(340aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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