AR09072PU-L Cyclophilin H / PPIH (1-177)

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0.5 mg / €1,000.00
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Cyclophilin H / PPIH

AR09072PU-L

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  • AR09072PU-L

Product Description for Cyclophilin H / PPIH

Cyclophilin H / PPIH.
Presentation: Purified

Properties for Cyclophilin H / PPIH

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms CYP20, CYPH, PPIase H, Peptidyl-prolyl cis-trans isomerase H, Rotamase H, Small nuclear ribonucleoprotein particle-specific cyclophilin H, SnuCyp-20, U-snRNP-associated cyclophilin SnuCyp-20, USA-CYP
Presentation Purified
Molecular weight 19.2 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O43447
Purity > 95 % by SDS PAGE
Background Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the U4/U6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors.
Concentration 1.0 mg/ml
Protocols Activity Assay
1.Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin.
2.Add 10 ul of recombinant Cyclophilin H (PPIH) protein with 1 ug in assay buffer.
3.Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4.Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5.Record the increase in A405 nm for 30 minutes at 25°C.
General Readings Reidt U., et al. (2000) J Biol Chem. 275(11):7439-42.
Horowitz DS., et al. (2002) EMBO 21(3):470-80.
Storage Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human cyclophilin H was expressed in E.coli and purified by conventional chromatography techniques.
AA Sequence:
MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM
Molecular weight:
(177 aa)
Specific activity:
> 220 nmoles/min/mg, defined as the amount of enzyme that cleaves
1 umole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin.
Format
Buffer System:
PBS, pH 7.4, 10% glycerol
Endotoxin level:
< 1.0 EU per 1 μg of protein (determined by LAL method)
Purity:
>95% by SDS PAGE
State:
Liquid purified protein
Purified
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