discontinued

PA184X Defensin beta 3

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Defensin beta 3

PA184X

Product Description for Defensin beta 3

Defensin beta 3.
Presentation: Purified

Properties for Defensin beta 3

Product Category Proteins & Growth Factors
Target Category
Quantity 20 µg
Synonyms BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3
Presentation Purified
Reactivity Bac
Molecular weight 5.1 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P81534
Purity > 98 % pure by SDS-PAGE and HPLC analyses
Add. information Centrifuge the vial prior to opening!
Background Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.
General Readings
  1. Sørensen OE, Thapa DR, Roupé KM, Valore EV, Sjöbring U, Roberts AA, et al. Injury-induced innate immune response in human skin mediated by transactivation of the epidermal growth factor receptor. J Clin Invest. 2006 Jul;116(7):1878-85. Epub 2006 Jun 15. PubMed PMID: 16778986. (Free PMC Article available, 9 images available)
  2. Binks MJ Attribution of the various inhibitory actions of the streptococcal inhibitor of complement (SIC) to regions within the molecule. Journal of Biological Chemistry, 2005, 280(20):20120-5.
  3. Miller LS, Sørensen OE, Liu PT, Jalian HR, Eshtiaghpour D, Behmanesh BE, et al. TGF-alpha regulates TLR expression and function on epidermal keratinocytes. J Immunol. 2005 May 15;174(10):6137-43. PubMed PMID: 15879109.
  4. Starner TD, Agerberth B, Gudmundsson GH, McCray PB. Expression and activity of beta-defensins and LL-37 in the developing human lung. J Immunol. 2005 Feb 1;174(3):1608-15. PubMed PMID: 15661923.
  5. Sørensen OE, Cowland JB, Theilgaard-Mönch K, Liu L, Ganz T, Borregaard N. Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors. J Immunol. 2003 Jun 1;170(11):5583-9. PubMed PMID: 12759437.
  6. Harder J, Schroder JM. RNase 7, a novel innate immune defense antimicrobial protein of healthy human skin. J Biol Chem. 2002 Nov 29;277(48):46779-84. Epub 2002 Sep 18. PubMed PMID: 12244054.
  7. Timothy D. Starner Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylation infection and immunity, 2002, Vol: 70 (9) pp. 5287-5289.
  8. Schibli DJ, Hunter HN, Aseyev V, Starner TD, Wiencek JM, McCray PB, et al. The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus. J Biol Chem. 2002 Mar 8;277(10):8279-89. Epub 2001 Dec 11. PubMed PMID: 11741980.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human BD-3 is a 5.1 kDa protein containing 45 amino acid residues.
AA Sequence:
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Biological activity:
Exhibits antimicrobial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E.coli.
Molecular weight:
(45 aa)
Format
Endotoxin level:
< 0.1 ng per µg of BD3 (1EU/µg)
Purity:
>98% pure by SDS-PAGE and HPLC analyses
State:
Lyophilized (0.2µ Sterile filtered) purified protein
Purified
Reconstitution:
Restore in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use.
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