SA6025X dnaK / Hsp70 (1-384)

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0.5 mg / €540.00
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dnaK / Hsp70

SA6025X

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  • SA6025X

Product Description for dnaK / Hsp70

dnaK / Hsp70.
Presentation: Purified

Properties for dnaK / Hsp70

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms Chaperone protein dnaK, Heat shock 70 kDa protein, Heat shock protein 70, MT0365, MTCY13E10.10, Rv0350
Presentation Purified
Molecular weight 41.6 kDa
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A6Y8
Purity > 95 % by SDS-PAGE
Background DnaK, originally identified for its DNA replication by bacteriophage λ in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.
Concentration 1.0 mg/ml
General Readings Zhu et al., (1996) Science 272, 1606-1614.
Storage Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
DnaK (amino acids 1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain (residues 385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques.
AA Sequence:
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTG
Molecular weight:
(384 amino acids)
Format
Buffer System:
25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM DTT, 10 %Glycerol
Purity:
>95% by SDS-PAGE
State:
Liquid purified protein
Purified
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