AR50097PU-S EIF3J / EIF3S1 (70-258, His-tag)

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50 µg / €370.00
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EIF3J / EIF3S1

Product Description for EIF3J / EIF3S1

EIF3J / EIF3S1.
Presentation: Purified

Properties for EIF3J / EIF3S1

Product Category Proteins & Growth Factors
Target Category
Quantity 50 µg
Synonyms Eukaryotic translation initiation factor 3 subunit 1, Eukaryotic translation initiation factor 3 subunit J, eIF-3-alpha, eIF3 p35
Presentation Purified
Molecular weight 24.0 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O75822
Purity > 90 % by SDS - PAGE
Background EIF3J (Eukaryotic translation initiation factor 3 subunit J) belongs to the EIF-3 subunit J family. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA entry channel of the 40S subunit, placing EIF3J directly in the ribosomal decoding center. EIF3J also interacts with eIF1A and reduces 40S subunit affinity for mRNA. A high affinity for mRNA is restored upon recruitment of initiator tRNA, even though EIF3J remains in the mRNA-binding cleft in the presence of tRNA.
Concentration 0.5mg/ml (determined by Bradford assay)
General Readings Fraser CS, et al. (2007) Mol Cell. 26(6):811-9.Block K.L., et al. (1998) J. Biol. Chem. 273:31901-31908
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human EIF3J protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MKISEKKKIA EKIKEKERQQ KKRQEEIKKR LEEPEEPKVL TPEEQLADKL RLKKLQEESD LELAKETFGV NNAVYGIDAM NPSSRDDFTE FGKLLKDKIT QYEKSLYYAS FLEVLVRDVC ISLEIDDLKK ITNSLTVLCS EKQKQEKQSK AKKKKKGVVP GGGLKATMKD DLADYGGYDG GYVQDYEDFM
Molecular weight:
(210aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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