Product Description for Epidermal Growth Factor (EGF)
Properties for Epidermal Growth Factor (EGF)
|Product Category||Proteins & Growth Factors|
|Molecular weight||6 kDa|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Proteins (de)|
|Purity||> 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
|Add. information||EGF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of human EGF as a Reference Standard.
|Background||Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
|Concentration||1 mg /ml|
|Storage||Lyophilized Epidermal Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution EGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.