discontinued

AR10035PU-L Epidermal Growth Factor Yeast

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1 mg / €360.00

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Epidermal Growth Factor Yeast

AR10035PU-L

Product Description for Epidermal Growth Factor Yeast

Epidermal Growth Factor Yeast.
Presentation: Purified

Properties for Epidermal Growth Factor Yeast

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6QBS2
Purity >95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Add. information Protein content:
Protein quantitation was carried out by two independent methods: 
1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 
2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.
Background

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. 
EGF (Epidermal Growth Factor) is polypeptide growth factor that stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human EGF is a 6.2 kDa protein containing 53 amino acid residues

Storage Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Source P. pastoris
Description
Description:
Epidermal Growth Factor Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa.
The EGF is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native EGF Human.
N-terminal Methionine has been completely removed enzymatically.
Biological activity:
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is 0.1 ng/ml corresponding to a specific activity of 1 x 107 Units/mg.
Molecular weight:
6 kDa
Format
Buffer System:
Each lyophilized mg contains contains 0.15M NaCl, 0.025M sodium bicarbonate, pH 7.5.
Purity:
>95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
State:
Sterile Filtered White lyophilized (freeze-dried) powder.
Purified
Reconstitution:
Restore the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm-1 H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
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