PA1013XC Fibroblast Growth Factor acidic (FGF-1)

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Fibroblast Growth Factor acidic (FGF-1)

Product Description for Fibroblast Growth Factor acidic (FGF-1)

Fibroblast Growth Factor acidic (FGF-1).
Presentation: Purified

Properties for Fibroblast Growth Factor acidic (FGF-1)

Product Category Proteins & Growth Factors
Quantity 1 mg
Synonyms FGF1
Presentation Purified
Molecular weight 17 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95 % Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE
Background Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Concentration 1 mg/ml (before lyophilization)
General Readings 1. Effects of a Single Percutaneous Injection of Basic Fibroblast Growth Factor on the Healing of a Closed Femoral Shaft Fracture in the Rat.
Calcif Tissue Int 2007 Jul 19;
2. Fibroblast growth factor-2 mediates transforming growth factor-beta action in prostate cancer reactive stroma.
Oncogene 2007 Jul 16;
3. Relevance of Partially Structured States in the Non-Classical Secretion of Acidic Fibroblast Growth Factor.
Biochemistry 2007 Jul 18;
4. Role of fibroblast growth factor 23 in phosphate homeostasis and pathogenesis of disordered mineral metabolism in chronic kidney disease.
Semin Dial 2007 Jul-Aug;20(4):302-8
5. Basic fibroblast growth factor modulates proliferation and collagen expression in urinary bladder smooth muscle cells.
Am J Physiol Renal Physiol 2007 Jul 18;
6. Human Fibroblast Growth Factor Receptor 1-IIIb Is a Functional Fibroblast Growth Factor Receptor Expressed in the Pancreas and Involved in Proliferation and Movement of Pancreatic Ductal Cells.
Pancreas 2007 Aug;35(2):147-157
Storage Lyophilized FGF-acidic although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution FGF-acidic should be stored at 4°C between 2-7 days and for future use below -18°C. Please avoid freeze-thaw cycles. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Source E. coli
Recombinant Human Fibroblast Growth Factor-acidic (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15803 Dalton.The acidic FGF is purified by proprietary chromatographic techniques. Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of FGF-acidic as a reference standard.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Asn-Leu-Pro
Biological activity:
The ED50 calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is less then 10 ng/ml.
Specific activity:
10e5 Units/mg
Buffer System:
Solution containing 10 mM Tris, pH 7.6 ,and 100 mM NaCl
>95% Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE
Sterile filtered white lyophilized powder
It is recommended to reconstitute the lyophilized FGF-acidic in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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