discontinued

PA1016 Fibroblast Growth Factor basic (FGF-2)

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10 µg / €110.00

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Fibroblast Growth Factor basic (FGF-2)

PA1016

Product Description for Fibroblast Growth Factor basic (FGF-2)

Fibroblast Growth Factor basic (FGF-2).
Presentation: Purified

Properties for Fibroblast Growth Factor basic (FGF-2)

Product Category Proteins & Growth Factors
Quantity 10 µg
Synonyms FGF2
Presentation Purified
Molecular weight 17 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 96 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of FGF-2 as a reference standard.
Background Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor.
It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
General Readings
  1. Exogenously added FGF-2 to NIH3T3 cells interacts with nuclear RSK2 in a cell cycle dependent manner. J Biol Chem 2005 May 6.
  2. Transendocardial and trans-epicardial intramyocardial FGF-2 administration: Myocardial and Tissue Distribution. Drug Metab Dispos 2005 May 6.
  3. Delatte M, Von den Hoff JW, Kuijpers-Jagtman AM. Regulatory effects of FGF-2 on the growth of mandibular condyles and femoral heads from newborn rats. Arch Oral Biol. 2005 Nov;50(11):959-69. PubMed PMID: 15878765.
  4. Naim R, Chang RC, Sadick H, Hormann K. Influence of hepatocyte growth factor/scatter factor (HGF/SF) on fibroblast growth factor-2 (FGF-2) levels in external auditory canal cholesteatoma (EACC) cell culture. In Vivo. 2005 May-Jun;19(3):599-603. PubMed PMID: 15875782.
  5. Gomide V, Chadi G. Glial bFGF and S100 immunoreactivities increase in ascending dopamine pathways following striatal 6-OHDA-induced partial lesion of the nigrostriatal system: a sterological analysis. Int J Neurosci. 2005 Apr;115(4):537-55. PubMed PMID: 15809219.
Storage Lyophilized FGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution FGF-2 should be stored at 4°C between 2-7 days and for future use
below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Shelf life: one year from despatch.
Description
Description:
Recombinant Human FGF-basic (FGF-2) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a Molecular Mass of 17353 Dalton. Recombinant FGF-2 is purified by proprietary chromatographic techniques.
AA Sequence:
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.
Biological activity:
The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is < 0.5 ng/ml.
Specific activity:
2 x 10e6 Units/mg
Format
Buffer System:
PBS, pH 7.4 without additives
Purity:
>96% pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
State:
White lyophilized (sterile filtered) powder
Purified
Reconstitution:
It is recommended to reconstitute the lyophilized FGF basic in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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