AR09172PU-N FKBP4 (1-459, His-tag)

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0.1 mg / €230.00
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Quick Overview

FKBP4

Product Description for FKBP4

FKBP4.
Presentation: Purified

Properties for FKBP4

Product Category Proteins & Growth Factors
Target Category
Quantity 0.1 mg
Synonyms 52 kDa FK506-binding protein, FK506-binding protein 4, FKBP52 protein, FKBP59, HBI, HSP-binding immunophilin, PPIase, Peptidyl-prolyl cis-trans isomerase, Rotamase, p59 protein
Presentation Purified
Molecular weight 53.9 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q02790
Purity > 90 % by SDS - PAGE
Background FKBP4, also known as FKBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes.
Concentration 1 mg/ml (determined by Bradford assay)
Protocols Activity Assay
1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 
2. Add 10 µl of recombinant FKBP4 protein with 1 µg in assay buffer.  
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 
4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 
5. Record the increase in A405 nm for 30 minutes at 25°C.
General Readings
  1. Ostrow KL., et al. (2009) Clin Cancer Res. 15(4):1184-91.
  2. Ma D., et al. (2008) J Biol Chem. 283(38):25963-70.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human FKBP4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA
Biological activity:
Specific Activity is > 300 nmoles/min/mg, defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH 8.0 using Chymotrypsin.
Molecular weight:
(479 aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl (pH 8.0) buffer containing 10% Glycerol
Endotoxin level:
< 1.0 EU per 1 µg of protein (determined by LAL method).
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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