discontinued

PA1020XC flt3-Ligand (flk2-Ligand)

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1 mg / €2,790.00

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flt3-Ligand (flk2-Ligand)

PA1020XC

Product Description for flt3-Ligand (flk2-Ligand)

flt3-Ligand (flk2-Ligand).
Presentation: Purified

Properties for flt3-Ligand (flk2-Ligand)

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Molecular weight 16 kDa
Source E. coli
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE
Background FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
General Readings 1. Prostate tumor microenvironment alters immune cells and prevents long-term survival in an orthotopic mouse model following flt3-ligand/CD40-ligand immunotherapy. J Immunother 2004 Jan-Feb;27(1):13-26
2. Impact of the tumor microenvironment on host infiltrating cells and the efficacy of flt3-ligand combination immunotherapy evaluated in a treatment model of mouse prostate cancer. Cancer Immunol Immunother 2003 Sep;52(9):535-45
3. Orthotopic treatment model of prostate cancer and metastasis in the immunocompetent mouse: efficacy of flt3 ligand immunotherapy. Int J Cancer 2003 Dec 10;107(5):773-80
4. Flt3 ligand: a novel cytokine prevents allergic asthma in a mouse model.
Int Immunopharmacol 2001 Nov;1(12):2081-9
5. Neutralization of IL-12 reverses rejection of mouse liver allografts from Flt3-ligand-treated donors and is associated with suppression of both cellular and humoral responses. Transplant Proc 2001 Feb-Mar;33(1-2):525
Storage Lyophilized FLT3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution FLT3 should be stored at 2-8°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Shelf life: one year from despatch.
Source E. coli
Description
Description:
Flt3-Ligand Mouse Recombinant produced in E.coli is non-glycosylated, polypeptide chain containing 144 amino acids. The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.Flt3-Ligand is purified by proprietary chromatographic techniques.
Biological activity:
The activity is determined by the ability to generate CD11c+ splenic dendritic cells in vivo in C57B1/6-12 week female mice. To obtain the desired affect, mice were treated with approximately 25ug of flt3-ligand once daily for 9 consecutive days via intraperitoneal injection.
Molecular weight:
16431 Dalton
Format
Purity:
>98% greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE
State:
Sterile filtered, white lyophilized powder without additives
Purified
Reconstitution:
It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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