PA1021XC flt3-Ligand (flk2-Ligand)

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flt3-Ligand (flk2-Ligand)


Product Description for flt3-Ligand (flk2-Ligand)

flt3-Ligand (flk2-Ligand).
Presentation: Purified

Properties for flt3-Ligand (flk2-Ligand)

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Molecular weight 18 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Background FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
General Readings
  1. Chan SS, Lyons N, McConnell I, Blacklaws BA. Cloning and sequencing of ovine Flt3 ligand. Int J Immunogenet. 2007 Jun;34(3):167-71. PubMed PMID: 17504506.
  2. Harada S, Kimura T, Fujiki H, Nakagawa H, Ueda Y, Itoh T, et al. Flt3 ligand promotes myeloid dendritic cell differentiation of human hematopoietic progenitor cells: possible application for cancer immunotherapy. Int J Oncol. 2007 Jun;30(6):1461-8. PubMed PMID: 17487367.
  3. Crucial role of FLT3 ligand in immune reconstitution following bone marrow transplantation and high dose chemotherapy. Blood 2007 Mar 22;
  4. Wils EJ, Braakman E, Verjans GM, Rombouts EJ, Broers AE, Niesters HG, et al. Flt3 ligand expands lymphoid progenitors prior to recovery of thymopoiesis and accelerates T cell reconstitution after bone marrow transplantation. J Immunol. 2007 Mar 15;178(6):3551-7. PubMed PMID: 17339451.
  5. Yoon WS, Choi WC, Sin JI, Park YK. Antitumor therapeutic effects of Salmonella typhimurium containing Flt3 Ligand expression plasmids in melanoma-bearing mouse. Biotechnol Lett. 2007 Apr;29(4):511-6. Epub 2007 Jan 19. PubMed PMID: 17235489.
  6. Bharadwaj AS, Agrawal DK. Flt3 ligand generates morphologically distinct semimature dendritic cells in ovalbumin-sensitized mice. Exp Mol Pathol. 2007 Aug;83(1):17-24. Epub 2006 Dec 19. PubMed PMID: 17182033. (Free PMC Article available, 6 images available)
Storage Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Flt3-Ligand should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E.coli.
Flt3-Ligand Human Recombinant produced in E.Coli is non-glycosylated, polypeptide chain containing 155 amino acids. Flt3-Ligand is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.
Biological activity:
The ED50 range=0.5-1.0 ng/mL, calculated by the dose-dependant stimulation of the proliferation of human OCMI-AML5 cells.
Molecular weight:
17605 Dalton.
Buffer System:
FLT-3 was lyophilized with no additives.
>98% Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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