Product Description for gldA
Properties for gldA
|Product Category||Proteins & Growth Factors|
|Synonyms||ECK3937, Glycerol dehydrogenase, JW5556|
|Molecular weight||41.1 kDa|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Proteins (de)|
|Purity||> 95 % by SDS - PAGE|
|Background||gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively.|
|Concentration||1.0 mg/ml (determined by bradford assay)|
|Protocols||1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 93mM Glycine, 93mM Potassium chloride, 2375mM Glycerol, 3mM b-NAD.2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.3. Add 20ul of recombinant gldA protein with various concentrations (0.2ug, 0.1ug, 0.05ug) in 180ul reaction buffer.4. Mix by inversion and record the decrease at A340nm for 10 minutes.|
|General Readings||Subedi K.P., et al. (2008) FEMS Microbiol. Lett. 279:180-187Gonzalez R., et al. (2008) Metab. Eng. 10:234-245|
|Storage||Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.