AR51950PU-N gldA (1-367, His-tag)

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0.1 mg / €370.00
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gldA

Product Description for gldA

gldA.
Presentation: Purified

Properties for gldA

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms ECK3937, Glycerol dehydrogenase, JW5556
Presentation Purified
Molecular weight 41.1 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A9S5
Purity > 95 % by SDS - PAGE
Background gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively.
Concentration 1.0 mg/ml (determined by bradford assay)
Protocols 1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 93mM Glycine, 93mM Potassium chloride, 2375mM Glycerol, 3mM b-NAD.

2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.

3. Add 20ul of recombinant gldA protein with various concentrations (0.2ug, 0.1ug, 0.05ug) in 180ul reaction buffer.

4. Mix by inversion and record the decrease at A340nm for 10 minutes.

General Readings Subedi K.P., et al. (2008) FEMS Microbiol. Lett. 279:180-187Gonzalez R., et al. (2008) Metab. Eng. 10:234-245
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE
Molecular weight:
(390aa), confirmed by MALDI-TOF
Specific activity:
> 14 Units/ml One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C
Format
Buffer System:
Phosphate buffered saline (pH7.4), 10% glycerol.
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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